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KMID : 0613820060160050740
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Journal of Life Science
2006 Volume.16 No. 5 p.740 ~ p.745
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Comparison of Genetic Diversity and Relationships of Genus Kalopanax Using ISSR Markers
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Huh Man-Kyu
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Abstract
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Inter simple sequence repeat (ISSR) markers were performed in order to analyse the phylogenetic relationships of four taxa of Castor-aralia (Kalopanax pictus): K. pictus, K. pictus var. magnificus, K. pictus var. maximowiczii, and thornless K. pictus. The 11 primers were produced 64 reproducible ISSR bands. Analysis of ISSR from individual plants of Korean K. pictus resulted in 41 polymorphic bands with 64.1%. When species were grouped by four taxa, within group diversity was 0.115 (HS), while among group diversity was 0.467 (GST) on a per locus basis. The estimated gene flow (Nm) for K. pictus var. maximowiczii and K. pictus var. magnificus were very higher than K. pictus. It is suggested that the isolation of geographical distance and reproductive isolation among K. pictus populations may have played roles in shaping the population structure of this species. In phenetic tree, ISSR markers are very effective in classifying natural populations as well as taxon levels of genus Kalopanax in Korea.
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KEYWORD
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Simple sequence repeat (ISSR), Kalopanax pictus
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